CryoEM: do you have any suggestion to concentrate samples after GRAFIX gradient?

After GRAFIX I am loosing all my sample on amicon filter.
Hello, loosing small quantities on amicon filters is quite common and dependant on the particular protein even huge amounts. Did you try other concentration methods ? Cryoconcentration is in my hands one of the best, but I do not know whether that will hamper your EM work afterwards. Best, Marcel 

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