what specific molecular pathways or immune cell subsets should researchers prioritize using flow cytometry to provide clinicians with a truly actionable, targeted immune signature of the patient?

it is a specific question that can help thousands for clinicians and lab experts to find targeted biomarkers that can achieve to diagnose and see a result for their therapies by blocking molecular pathways that can find the biochemical, environmental tumor microenvironment,  multiplication, resistance of the tumor and many others and will help to block all mechanisms of the carcinogenesis

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建议流式检测聚焦于PD-1/LAG-3双阳性耗竭T细胞比例、M-MDSC与CD8⁺T细胞比值以及CD127/KLRG1记忆/终末分化平衡,这三项指标可形成指导联合免疫治疗和预测耐药的可操作免疫特征。