Results
(9 Answers)

  • Expert 9

    Laboratory reagents, equipment, PPE, and air. Standard practices to minimise contamination should include avoidance (or minimisation when not possible) of plastic equipment (use glass or metal instead). Also consider conducting work in a clean air hood with ideally (thought noting cost implications) working in a clean room with filtered air. Use of cotton lab coats should be obligatory, as is regular, frequent (e.g. daily) washing of lab surfaces with ethanol. 
  • Expert 3

    The major sources of contamination are fibers, usually from clothing, but also from air conditioning units. Other sources include plastic labware, contaminated reagents,  packaging materials. Minimal sources could include cross contamination from prior analyses. Minimize the use of plasticware, use of natural fibers (e.g., cotton), use of safety cabinets, filter reagents, clean surfaces and material rinsing are all recommended practices. 
  • Expert 2

     The MNP contamination has multiple sources contributing to background interference in major sensitive analyses. The major sources are coming from airborne fibers that could come from synthetic lab coats and also from dust from plastic equipment, shedding from plastic labware and consumables, contaminated water systems such as ultrapure or Milli-Q sources, and contact with plastic-based surfaces and instruments. More than that, the  human contamination  that could arise from clothing, hair, and improper handling also poses a risk. In order to be able to minimize these contaminants,  the laboratories should implement standard practices such as working in clean air environments, using glass or metal equipment where possible, and filtering all reagents and water. Complementary measures could include the incorporation of procedural blanks, switch to the use of cotton-based PPE,  the regularly cleaning  of the surfaces with filtered solvents, monitoring  the environmental contamination, and minimizing sample exposure to open air. 
  • Expert 4

    In principal, anything that is present in the laboratory can serve as a source for background MNP contamination unless it has been verified that this is not the case. This ranges from solvents, any lab equipment, ceiling materials, staff working in the laboratory, etc.
    The key issue is that laboratory staff and management need to have a constant focus on possible sources of contamination and on minimizing MNP contamination. This is comparable to working in a clean room, and it is the mindset that is in itself the key issue.
    A required standard practise is, apart from technical efforts like including blanks, to maintain the mindset and take the appropriate precautions. One of the issues is in this respect the need to critical inventorize possible sources and actively secure the mindset.
  • Expert 5

    Ambient air is a major source of contamination. It contains MNP from all the plastic sources inside and nearby outside of the laboratory. As stated, laminar flow hoods help. 
    Samples can be contaminated even if they are "covered". Covering that are not air tight will allow the sample containers to "breathe" as the laboratory temperature cycles during the day/night. This will bring MNP into the stored samples.  Recommended: airtight storage containers kept in refrigerator or freezer which limits magnitude of temperature cycles.
  • Expert 6

    Major laboratory sources for background MNP contaminations are containers, tools, instruments, and PPEs made of synthetic polymers. Standard practices are to use alternative materials such as metal and glasses and use dedicated area and setups and MNP study.
  • Expert 8

    Dust, in general, is the biggest source and most of the microplastics in dust of a typical lab will come from clothing. 
    opening samples only inside hepa filtered air
    nonplastic clothing or labcoats
    minimum or no use of gloves
    filtering all reagents
    cleaning glassware and metal instruments thoroughly - rinsing with <1um filtered water and baking them at 400 oC or higher
    keeping clean glassware covered with baked foil
    general lab cleanliness (wet dusting and vacuuming floors when samples are NOT being processed)
  • Expert 7

    Major laboratory sources for background MNP contamination are air, reagents, equipment, glassware, containers, vial caps, gloves, lab coats, filters.
    Select stearate free gloves; cover the samples; use solvent cleaned glassware or metal; be cautious of the cap materials as most caps contain plastics and should chose those without targeted analytes.
  • Expert 1

    Although not discussed in the manuscript, one major source of contamination is sampling devices and contamination while sampling biospecimens.  Most studies that analyzed human specimens such as blood, brain, and placenta received samples from physicians or collaborators who collected samples.  The MNP levels reported in those studies were <10 particles per tissue or per unit mass.  However, such studies can be sensitive and often receive media.  Data quality of those studies needs to be strictly validated.  Those samples were collected in an environment that could easily contaminate blood or placenta tissues (in hospital settings where physicians use plastic products during surgery).  It is important that study investigators who are familiar with such contamination issues should be involved in sample collection.  

    Establishing a lab environment that is free of MNPs is very important.  A difficult source of avoid MNP contamination is airborne particles.  Controlling/monitoring lab air quality is critical and ISO clean 5 or class 6 clean rooms may help reduce contamination by airborne particles, but such infrastructure can be expensive.  This is critical for labs measuring MNPs at trace levels, especially in human specimens.  A clean room, free of plastics, should be used in sample preparation.  Products including collection and storage devices, sample extraction tools, that come into contact with samples should be free of plastics.

Laboratory sources of microplastic (MNP) contamination include airborne fibers (mentioned by 6 experts), clothing/PPE (5 experts), plastic labware/equipment (5 experts), reagents (3 experts), and sampling devices (1 expert).

Recommended practices to minimize contamination:

  • Use alternative materials: Replace plastic with glass or metal equipment (Experts 2, 3, 6, 7, 9)
  • Control air quality: Work in clean air hoods, HEPA-filtered environments, or clean rooms (Experts 2, 5, 8, 9)
  • Use cotton-based PPE: Cotton lab coats instead of synthetic materials (Experts 2, 8, 9)
  • Filter reagents and water: Ensure all liquids are filtered (Experts 2, 3, 8)
  • Regular cleaning: Clean surfaces with filtered solvents/ethanol (Experts 2, 8, 9)
  • Airtight storage: Keep samples in sealed containers (Expert 5)

Expert 4 uniquely emphasized the importance of mindset and awareness as critical to contamination control, while Expert 1 highlighted the need for contamination control during initial sample collection, especially for human specimens.

Summary Generated by AI
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Expert 9
07/28/2025 07:05
The reviewers are pretty consistent in their views and cover the potential sources of contamination well.
1 vote 1 0 votes
Expert 8
07/31/2025 01:46
I agree with all comments and especially want to echo what expert 1 discusses before and during collection of human samples. This environment is not the laboratory where the samples will be processed and analyzed and will be less controlled for microplastic contamination.  It is key to mention this in the paper and suggest best practices that will control, minimize, cut away, or quantify the background contamination in room for surgery prep and performance.
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Expert 4
07/31/2025 02:18
The comments provide a good addition to what is already written in the manuscript. I agree with the comments and especially like the overall nominator of dust being the major source.
1 vote 1 0 votes
Expert 3
07/31/2025 03:50
Expert 1 points to an important issue. In fact, many biological samples, namely, human samples, are collected and stored resorting to plastic material and it is quite difficult to determine the source of any micro- and nanoplastics found. For the materials used, chemical signatures may be used, but MPs and NPs present due to contamination are harder to trace. This is particular evident for "legacy samples", which have been stored for long time, sometimes, years, and are then explored for the presence of MPs. But, given the these were not collected and handled in accordance with MP/NP analyses guidelines, it is quite difficult to definitively ascertain the origin of any putative MPs. 
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Expert 7
07/31/2025 08:18
I suggest that the author review the above comments and make slight modifications to incorporate some of the valuable points raised by the evaluators.
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Expert 5
07/31/2025 10:08
Because of the ubiquity of MNP in our lives, one must envision every environment the samples may pass through or reside in. The reviewers have mentioned many sources of contamination, but the section title "...major laboratory sources for background MNP contamination..." constrains thinking to the analysis laboratory. As reviewers point out, the sampling environment, and storage environment are also a source on MNP contamination. One must not forget sample transport, sample "log-in", laboratory storage before processing, etc. One must envision themselves as a MNP and follow the entire life of the particle facilitate envisioning possible sources of contamination that need to be risk evaluated.
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Expert 5
07/31/2025 10:17
If the expense of a "clean room" and "clean scientists" if prohibitive, background MNPs in the laboratory may be reduces with the utilization of residential/commercial "air purifiers." Units equipped with HEPA 14 filters will filter 99.995% of 0.3 - 0.1 micron particles from 186 sq meters (2,000 sq ft). 
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